During chip preparation, the micro-channels are filled with a sieving polymer and fluorescence dye. Once the wells and channels are filled, the chip becomes an integrated electrical circuit. The pin electrodes of the cartridge are arranged so that they fit into the wells of the chip. Each electrode is connected to an independent power supply that provides maximum control and flexibility.
Charged biomolecules like DNA or RNA are electrophoretically driven by a voltage gradient-similar to slab gel electrophoresis. Because of a constant mass-to-charge ratio and the presence of a sieving polymer matrix, the molecules are separated by size. Smaller fragments are migrating faster than larger ones. These complexes are detected by laser-induced fluorescence. To arrange a training please place a training request through PPMS. The Bioanalyzer training takes about 1. Only one person will be trained at a time to fully experience the hands-on training.
We highly recommend bringing your own samples to the training to generate useful data. Alternatively you are welcome to bring your own reagents. Agilent Bioanalyzer The Agilent Bioanalyzer uses a micro-capillary based electrophoretic cell that allows rapid and sensitive investigation of nucleic acid samples. High resolution of a large number of bands — critical for any multiplex PCR application. Broad linear dynamic range — enables the detection of less abundant products, e.
Ready-to-use — standardized assays and pre-packaged reagent kits. The microfluidic, electrophoresis system needs minimal sample usually 1ul to carry out assays. The Agilent Expert Software allows data to be reported in multiple formats including.
The Bioanalyzer can be used to assess the size and quality of DNA over a range of sizes and concentrations.
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